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目的通过对2009年中国(未包括香港、澳门特别行政区和台湾地区,下同)脊髓灰质炎(脊灰)实验室网络(Polio Laboratory Network,PLN)监测数据进行分析,评估其运转情况,为中国维持无脊灰状态提供病毒学依据。方法分析中国免疫规划监测信息管理系统数据库中,31个省(自治区、直辖市,下同)上报的急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例个案调查表和中国疾病预防控制中心(Center for Disease Control and Prevention,CDC)病毒病预防控制所国家脊灰实验室(National Polio Laboratory,NPL)的监测数据库,评价PLN的各项运转指标。结果 2009年全国PLN共收集了4829例AFP病例的9621份粪便标本,14d内双份粪便标本采集率为90.8%,合格粪便标本采集率为90.0%。按世界卫生组织(World Health Organization,WHO)第4版《脊灰实验室手册》的要求进行病毒分离和鉴定,试验结果28d内及时反馈率为95.4%。2009年从158例AFP病例中分离到脊灰病毒(Poliovirus,PV),分离率为3.29%;607例分离到非脊灰肠道病毒(Non-polio Enterovirus,NPEV),分离率为12.63%。2009年,NPL收到PLN送检的214株PV阳性分离物,对284株单血清型PV采用VP1编码区核苷酸序列测定与分析的方法进行型内鉴定,发现1株Ⅱ型疫苗衍生脊灰病毒,未发现脊灰野病毒。2009年,NPL以优异的成绩通过了WHO盲样标本的能力验证和现场认证考核;31个省级CDC脊灰实验室通过了与NPL相同的盲样标本的能力验证;12个省级CDC脊灰实验室通过了WHO的实验室现场评估考核。结论 2009年,PLN运转正常、敏感有效,为维持无脊灰状态提供了病毒学依据,中国继续保持无脊灰状态。
Objective To analyze the monitoring data of the Polio Laboratory Network (PLN) in China in 2009 (excluding Hong Kong, Macau Special Administrative Region and Taiwan) To maintain polio-free status provides virological basis. Methods A case history of Acute Flaccid Paralysis (AFP) reported by 31 provinces (autonomous regions and municipalities directly under the Central Government) and China Center for Disease Control and Prevention (AFP) was analyzed in the database of China Immunization Program Monitoring Information Management System. Control and Prevention, CDC) Virus Pollution Prevention and Control Institute National Polio Laboratory (NPL) monitoring database to evaluate various indicators of PLN operation. Results In 2009, a total of 9621 stool specimens of 4829 cases of AFP were collected in the national PLN. The stool samples collected in double stool samples were 90.8% in 14 days and 90.0% in qualified stool samples. Virus isolation and identification were performed according to the “Polio Laboratory Manual” of the 4th edition of the World Health Organization (WHO). The response rate was 95.4% in 28 days. Poliovirus (PV) was isolated from 158 cases of AFP in 2009 with the isolation rate of 3.29%. Non-polio Enterovirus (NPEV) was isolated from 607 cases with isolation rate of 12.63%. In 2009, NPL received 214 PV-positive isolates from PLN for in-vivo identification of 284 single-serotype PVs using the VP1 coding region nucleotide sequence analysis and analysis. One type II vaccine-derived derivative Gray virus, no wild polio virus found. In 2009, NPL passed the proficiency testing and on-the-spot certification examinations of the blind samples of the WHO with excellent results; 31 provincial CDC polio laboratories passed the capacity verification of the same blind samples as the NPL; 12 provincial CDC ridges Ash Laboratory has passed the WHO laboratory site assessment assessment. Conclusion In 2009, PLN was functioning normally and sensitively, providing a virological basis for maintaining polio-free status. China continued to remain polio-free.