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目的:探讨中晚期胃癌组织中ABCG2和P-gp蛋白的表达及其临床意义。方法:采用免疫组化法检测60例中晚期胃癌组织和对应的癌旁正常组织中ABCG2和P-gp蛋白的表达情况,分析其与临床病理特征的关系。结果:ABCG2主要定位于细胞膜,胞质中亦有表达,P-gp定位于细胞膜和细胞质,以细胞质为主。ABCG2和P-gp在胃癌组织中的阳性表达率分别为95.0%(57/60)和85.0%(51/60),显著高于癌旁正常组织的81.7%(49/60)和25.0%(15/60),χ2值分别为12.264和52.286,P值分别为0.007和0.000;二者在癌中的共表达率为81.7%(49/60),在表达强度上差异有统计学意义(χ2=28.381,P=0.000),且呈正相关(r=0.156),但差异无统计学意义,P=0.233;在表达程度上一致性差(Kappa=0.084),且差异无统计学意义,P=0.282;其表达均与肿瘤TNM分期、分化状态、浸润深度密切相关(P值均=0.000),与患者年龄、性别、肿瘤大小、有无淋巴结和远处转移无关,P值均>0.05。结论:ABCG2与P-gp作为胃癌检测中的敏感指标,可能成为胃癌诊断、恶性程度、肿瘤进展、耐药、疗效及预后的新指标。
Objective: To investigate the expression of ABCG2 and P-gp protein in advanced gastric cancer and its clinical significance. Methods: The expressions of ABCG2 and P-gp proteins in 60 cases of advanced gastric cancer tissues and corresponding normal tissues were detected by immunohistochemistry. The relationship between ABCG2 and P-gp protein expression was analyzed with clinicopathological features. Results: ABCG2 mainly located in the cell membrane and cytoplasm. P-gp was localized in the cell membrane and cytoplasm, mainly cytoplasm. The positive rates of ABCG2 and P-gp in gastric cancer tissues were 95.0% (57/60) and 85.0% (51/60), respectively, which were significantly higher than those in normal tissues (81.7%, 49/60) and 25.0% 15/60). The χ2 values were 12.264 and 52.286, respectively. The P values were 0.007 and 0.000, respectively. The co-expression rates of the two in cancer were 81.7% (49/60), with significant difference in expression intensity (χ2 = 28.381, P = 0.000). There was a positive correlation between them (r = 0.156), but the difference was not statistically significant (P = 0.233) (P = 0.000) .There was no correlation between TNM stage and tumor size, lymph node metastasis and distant metastasis (P> 0.05). Conclusion: ABCG2 and P-gp as sensitive indicators in gastric cancer detection may be a new indicator of gastric cancer diagnosis, malignancy, tumor progression, drug resistance, efficacy and prognosis.