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目的:辛夷挥发油灌胃给予小鼠后的急性毒性试验。方法:按改良寇氏法设计试验,观察辛夷挥发油单次给药的毒性反应和死亡情况,测定其半数致死量(LD50)。结果:辛夷挥发油单次灌胃给药的LD50为7.11mL·kg-1,LD50的95%可信限为6.08～8.31mL·kg-1,95%平均可信限为(7.1±1.1)mL·kg-1。对死亡小鼠剖解后肉眼观察心、肝、肾、肺、脑、脾、胸腺等重要器官,无明显异常发现。结论:辛夷挥发油具有一定的毒性,为其进一步的研发应用提供了必要的参考。
Objective: Xiyi volatile oil after intragastric administration of acute toxicity test in mice. Methods: According to the modified Kovar test, the toxicity and mortality of single dose of volatile oil of Magnolia biondii were observed. The LD50 was determined. Results: LD50 of a single intragastric administration of volatile oil of Magnolia biondii was 7.11 mL · kg-1, the 95% confidence interval of LD50 was 6.08 ~ 8.31 mL · kg-1, and the average confidence limit was 7.1 ± 1.1 mL · Kg-1. The necrotic mice were dissected and macroscopically observed in important organs such as heart, liver, kidney, lung, brain, spleen and thymus without obvious abnormal findings. Conclusion: Volatile oil of Magnolia biondii has certain toxicity, which provides the necessary reference for its further research and development.