论文部分内容阅读
以低密度脂蛋白（ＬＤＬ）为阿克拉霉素（ＡＣＭ）的载体，以游离ＡＣＭ为对照，体外研究ＬＤＬ－ＡＣＭ复合物对白血病细胞株Ｋ５６２的细胞毒作用及其影响因素。结果显示：（１）Ｋ５６２细胞对ＬＤＬ－ＡＣＭ复合物摄取较游离ＡＣＭ明显增加，在前３０ｍｉｎ摄取较快，随时间延长摄取逐渐减慢；（２）过量的天然ＬＤＬ、肝素及低温均可使细胞摄取复合物明显减少，而过量的ＨＤＬ则无影响，去脂蛋白血清预刺激的细胞摄取复合物明显增多；（３）对Ｋ５６２细胞的细胞毒作用随复合物浓度增加而逐渐增强，达１０ｍｇ／Ｌ以上时，增加药物浓度其细胞毒作用不再加强。
Using low-density lipoprotein (LDL) as the carrier of aclarubicin (ACM) and free ACM as control, the cytotoxicity of LDL-ACM complex on leukemia cell line K562 and its influencing factors were studied in vitro. The results showed that: (1) The uptake of LDL-ACM complex in K562 cells was significantly higher than that in free ACM. The uptake in K562 cells was faster in the first 30 minutes, and it gradually slowed down as time went by; (2) Excessive natural LDL, heparin, and low temperature all contributed to the increase. Cell uptake complexes were significantly reduced, while excess HDL had no effect, and cell uptake complexes pre-stimulated with apolipoprotein serum increased significantly; (3) Cytotoxicity on K562 cells increased gradually with increasing concentrations of complexes up to 10 mg Above /L, the cytotoxic effect is no longer enhanced with increasing drug concentration.