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为比较水温对没食子酸与恩诺沙星联合用药后恩诺沙星在福瑞鲤体内的药代动力学及其残留消除规律的影响,采用对照试验,将福瑞鲤随机分为高温组(26±1)℃和低温组(20±1)℃,没食子酸与恩诺沙星按质量比10∶1配伍后单次混饲口灌给药,用量为体质量2 mg/kg(以恩诺沙星计),血浆、肌肉、肝胰脏、肾脏各组织样品中的恩诺沙星含量经高效液相色谱法测定。数据经DAS 3.0药物代谢动力学软件的非房室模型统计矩方法分析。结果表明:恩诺沙星在血浆中高温组达峰时间T_(max)(0.75 h)早于低温组(4 h),且高温组达峰浓度C_(max)、药时曲线下面积AUC、表观分布容积Vz均大于低温组,分别为1.966 mg/L、130.122 mg/(L·h)、16.9 L/kg和1.610 mg/L、102.795 mg/(L·h)、3.186 L/kg。在鱼体肌肉、肝胰脏、肾脏中的药物浓度均呈现先升高后降低的变化趋势。高温组各组织中的药物在达峰前均高于低温组相应组织含量,而达峰后,高温组较低温组消除更快。表明水温对与没食子酸联用后恩诺沙星的吸收、分布、消除代谢均有较大促进作用。
To compare the effects of water temperature on the pharmacokinetics and residual elimination of enrofloxacin after the combination of gallic acid and enrofloxacin in Fukui carp, a control experiment was conducted to divide the fry carp into high temperature group 26 ± 1) ℃ and low temperature group (20 ± 1) ℃. When the ratio of gallic acid to enrofloxacin was 10:1, the dosage was 2 mg / kg body weight Norfloxacin), and the contents of enrofloxacin in plasma, muscle, hepatopancreas, and kidney tissue samples were determined by high performance liquid chromatography. Data were analyzed by non-compartmental model moment of approximation using the DAS 3.0 pharmacokinetic software. The results showed that the peak time of enrofloxacin in plasma was higher than that in the hypothermia group (T hmax 0.75 h), and the peak concentration C max of the enrofloxacin group, AUC, The apparent volume of distribution, Vz, was significantly higher than those in the low-temperature group (1.966 mg / L, 130.122 mg / (L · h), 16.9 L / kg and 1.610 mg / L, 102.795 mg / (L · h) and 3.186 L / kg, respectively) In fish muscle, liver, pancreas, kidney drug concentration showed the first increase and then decrease the trend. The high-temperature group in each organization before the peak drug levels were higher than the corresponding content of the low-temperature group, and reached the peak, the high-temperature group than the low-temperature group to eliminate faster. Show that the water temperature on the gallic acid combined with enrofloxacin absorption, distribution, elimination of metabolism have a greater role in promoting.