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某些专性厌氧菌对氨基糖苷类抗菌素具有天然的耐药性。为了探讨这种耐药机制,本文作者选取了两株专性厌氧菌(产气荚膜杆菌UACp—1、脆弱类杆菌UABf—1)进行了最小抑菌浓度(MIC)、无细胞系统氨基酸的掺入、抗菌素与核蛋白体结合力、钝化酶、抗菌素进入菌细胞的积累量以及菌体内醌类和细胞色素等项目的检测。在一些项目的检测中,还采用了大肠杆菌K12SA1306及其耐链霉素变种SA1306Strr供实验的对照。测定MIC时,采用脑浸液肉汤(BHIB)及加有补充物(二甲萘醌、高铁血红素)的BHISB;另外还采用Haaris和Reddy的半合成培养基及加有补充物(高铁血红素、二甲萘醌及延胡索酸)的半合成培基。在厌氧条件下,兼用试管法和纸片进行检测。实验结果证明:产气荚膜杆菌,在四种培基中对
Some obligate anaerobic organisms have natural resistance to aminoglycoside antibiotics. In order to explore this resistance mechanism, the authors selected two obligate anaerobic bacteria (Clostridium perfringens UACp-1, Bacteroides fragilis UABf-1) for the minimum inhibitory concentration (MIC), cell-free system amino acids The incorporation of antibiotics and ribosomes, the inactivation of enzymes, the amount of antibiotics entering the bacterial cells, and the detection of quinones and cytochromes in the bacteria. In some tests, Escherichia coli K12SA1306 and its streptomycin-resistant variety SA1306Strr were also used as controls. For MIC determination, BHISB supplemented with mannose broth (BHIB) and mannose supplemented with menaquinone (heme) were used. In addition, Haaris and Reddy semi-synthetic medium supplemented with supplements (methemoglobin Mannitol, melem, mannitol, and fumarate). Under anaerobic conditions, both test tubes and paper for testing. The experimental results show that: C. perfringens, cultured in four pairs of medium