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【目的】观察3种化痰方剂(黄连温胆汤、瓜蒌薤白半夏汤、二陈汤)含药血清超滤组分对人白血病单核细胞(THP-1)源性巨噬细胞活性及其泡沫化的影响。【方法】以佛波酯(PMA)诱导THP-1为巨噬细胞,经氧化低密度脂蛋白(ox-LDL)处理建立动脉粥样硬化泡沫细胞模型;制备3种化痰方剂含药血清及空白血清经10 KD超滤器超滤后,作用于泡沫细胞模型。采用四甲基偶氮唑盐(MTT)法及油红染色观察各组对巨噬细胞存活率和泡沫化的影响;通过流式细胞术检测各组对巨噬细胞凋亡率的影响。【结果】THP-1经100 nmol/L PMA诱导24 h后,与50μg/m L ox-LDL共孵育24 h,获得泡沫细胞模型。黄连温胆汤含药血清超滤成分作用于巨噬细胞,可明显提高存活率,抑制泡沫化,降低细胞凋亡率。二陈汤和瓜蒌薤白半夏汤含药血清超滤成分可增加泡沫化,未见显著抗凋亡作用。【结论】黄连温胆汤具有抗动脉粥样硬化作用,可能与其能抗巨噬细胞凋亡,降低巨噬细胞脂质吞噬作用相关;二陈汤和瓜蒌薤白半夏汤可能具有提高巨噬细胞脂质吞噬功能的作用。
【OBJECTIVE】 To observe the effect of serum-containing ultrafiltration components of three kinds of Huatan prescriptions (Huanglian Wendan Decoction, Guluo Baibanxia Decoction and Erchen Decoction) on the activity of THP-1-derived macrophages And its bubble effect. 【Method】 THP-1 was induced by phorbol ester (PMA) as macrophages and oxidized low-density lipoprotein (ox-LDL) was used to establish the model of atherosclerotic foam cells. Three serums containing Huatan prescription were prepared, After the blank serum was ultrafiltrated by 10 KD ultrafilter, it was applied to the foam cell model. The effects of each group on macrophage survival rate and foam formation were observed by MTT method and oil red staining. The effect of each group on the rate of macrophage apoptosis was detected by flow cytometry. 【Results】 THP-1 cells were incubated with 50 μg / mL ox-LDL for 24 h after being induced by 100 nmol / L PMA for 24 h. The foam cell model was obtained. The action of the Huanglian Wendan Decoction containing serum on the macrophages can obviously improve the survival rate, inhibit the foaming and reduce the apoptosis rate. Two Chen Tang and melon 蒌 薤 white Pinellia decoction serum containing ultrafiltration ingredients can increase the foam, no significant anti-apoptotic effect. 【Conclusion】 Huanglian Wendan Decoction has anti-atherosclerotic effect, which may be related to its ability to resist macrophage apoptosis and reduce lipid phagocytosis of macrophages; Erhuan Decoction and Guizhiloba Banxiaxie Decoction may have the effects of increasing macrophage Cell lipid phagocytosis function.