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目的研究Ｅ－选择素不同结构域缺失对其粘附功能的影响。方法从人胎脐静脉内皮细胞总ＲＮＡ中用ＲＴ－ＰＣＲ方法获其全长ｃＤＮＡ，设计并合成三对缺失Ｅ－选择素分子的ＥＧＦ结构域及ＥＧＦ＋ＣＲ１＋ＣＲ２结构域引物，并将三者基因分别在痘苗病毒真核表达系统中表达。结果细胞粘附功能试验结果表明，Ｅ－选择素分子ＥＧＦ结构域或ＥＧＦ＋ＣＲ１＋ＣＲ２结构域的缺失削弱了Ｅ－选择素分子的粘附能力。此外，本研究尚克隆并在痘苗表达系统中表达了可溶性Ｅ－选择素分子。结论Ｅ－选择素分子不同结构域缺失对其粘附能力削弱现象的发现为临床某些疾病的发病机理揭示及治疗提供了线索。可溶性Ｅ－选择素分子在痘苗病毒表达系统中表达成功为与其介导的白细胞粘附、肿瘤转移等过程的调节奠定了物质基础
Objective To investigate the effect of different domains of E-selectin on adhesion function. Methods The full-length cDNA was obtained from total RNA of human fetal umbilical vein endothelial cells by RT-PCR. Three pairs of EGF domain and EGF + CR1 + CR2 domain primers were designed and synthesized. Vaccinia virus eukaryotic expression system. Results The cell adhesion function test results showed that the deletion of the E-selectin EGF domain or the EGF + CR1 + CR2 domain impaired the adhesion of E-selectin molecules. In addition, the present study cloned and expressed soluble E-selectin molecules in a vaccinia expression system. CONCLUSION: The finding that the deletion of different domains of E-selectin on its adhesion abilities has provided clues to the pathogenesis and treatment of some diseases in clinical practice. The successful expression of soluble E-selectin in vaccinia virus expression system lays the material foundation for the regulation of the process of leukocyte adhesion, tumor metastasis and so on