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细胞分裂素氧化酶降解细胞分裂素,从而调控植物的生长发育,克隆和分析玉米细胞分裂素氧化酶基因(Zm CKX),为研究Zm CKX在玉米抗逆反应中的作用奠定基础。运用RT-PCR和RACE技术克隆了Zm CKX基因的c DNA全长,并对其进行了初步的生物信息学分析。琼脂糖凝胶电泳鉴定结果表明,PCR扩增出一条约1035 bp的片段,与目的片段大小相似。将目的片段与p MD-19t构建的p MDZm CKX重组质粒并转化为DH5α,经检测片段与原始片段大小相同。Blastn分析结果表明,其与Zm CKX1的相似性为99%。对该基因序列分析表明,Zm CKX基因编码的蛋白的开放阅读框为1035 bp,起始位点为23 bp,终止位点为1057 bp。Zm CKX基因长1035 bp,编码344个氨基酸。它与Zm CKX1基因核苷酸同源性99%。Zm CKX基因编码的蛋白是存在于细胞质中的亲水蛋白。
Cytokinin oxidase degrades cytokinin to regulate plant growth and development, cloning and analysis of maize cytokinin oxidase gene (Zm CKX), which laid the foundation for the study of Zm CKX in maize anti-retrogradation. The full-length c DNA of Zm CKX gene was cloned by RT-PCR and RACE, and its bioinformatics analysis was carried out. The results of agarose gel electrophoresis showed that a 1035 bp fragment was amplified by PCR, which was similar to the fragment of interest. The recombinant plasmid p MDZm CKX constructed with p MD-19t was transformed into DH5α, and the size of the fragment was the same as that of the original fragment. Blastn analysis showed that the similarity with Zm CKX1 was 99%. Sequence analysis of this gene showed that the open reading frame of the protein encoded by Zm CKX gene was 1035 bp, with a start site of 23 bp and a stop site of 1057 bp. The Zm CKX gene is 1035 bp in length and encodes 344 amino acids. It has 99% nucleotide homology with the Zm CKX1 gene. The protein encoded by the Zm CKX gene is a hydrophilic protein present in the cytoplasm.