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目的:探讨补肾健脾活血方对骨质疏松骨骼肌线粒体通透转换孔(MPTP)的调控作用。方法:将6月龄的雌性SD大鼠随机分为手术模型组、雌激素组、中药组和空白对照组。以双侧卵巢摘除手术造模成功后,中药组给予中药灌胃,每天1次;雌激素组予尼尔雌醇灌胃,每周1次;手术模型组和空白对照组给予蒸馏水灌胃,每天1次。治疗3月后,收集肌肉组织进行MPTP的活性检测。结果:MPTP吸光值(OD)比值在30、60min这两个时间点上,雌激素组、空白对照组与手术模型组比较,差异均有显著性意义(P<0.05);而中药组与其他各组比较,差异均无显著性意义(P>0.05)。结论:补肾健脾活血中药可在一定程度上调控骨质疏松骨骼肌MPTP的开放程度,但药物浓度未达到作用的阀值,差异无显著性意义,值得进一步研究。
Objective: To investigate the regulation effect of Bushen Jianpi Huoxue Fang on osteoporotic skeletal muscle mitochondrial permeability transition pore (MPTP). Methods: Six-month-old female Sprague-Dawley rats were randomly divided into operation model group, estrogen group, traditional Chinese medicine group and blank control group. After successful ovariectomy surgery, the traditional Chinese medicine group was given gavage once a day, while the estrogen group was given gavage once a week. The model group and the blank control group were given distilled water, 1 day. After 3 months of treatment, muscle tissue was collected for MPTP activity testing. Results: Compared with the model group, the difference of MPTP OD value between 30 and 60min was significant (P <0.05), while the difference between the MPTP group and the control group was significant There was no significant difference between groups (P> 0.05). Conclusion: Bushen Jianpi Huoxue Chinese medicine can to some extent regulate the openness of MPTP in osteoporotic skeletal muscle, but the drug concentration does not reach the threshold of the effect, the difference was not significant, worthy of further study.