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采用体外震波碎石机对家兔肝脏实施震波冲击实验,并用硝酸镧示踪和碱性磷酸酶细胞化学亚显微定位技术对兔肝组织进行电镜研究。观察显示:实验组肝见镧颗粒穿过细胞膜进入胞质,并围绕线粒体呈颗粒状沉积或选择性地进入线粒体;毛细胆管腔及紧密联接区和肝细胞间隙均有镧颗粒沉积,紧密联接区及肝细胞间隙亦见异常的碱性磷酸酶反应物。超微结构示线粒体肿胀、膜破坏及内质网扩张。结果表明,震波作用的靶细胞单位是膜结构和线粒体,其早期损伤效应是细胞膜性结构的通透性增高及完整性的丧失,进而导致亚细胞结构改变和生化的异常。
Shock wave experiments were performed in rabbit liver by using an extracorporeal shock wave lithotripsy machine. The liver tissues of rabbits were studied by electron microscopy with lanthanum nitrate tracing and alkaline phosphatase cytochemical sub-microscopy. The observation showed that the hepatic lanthanum particles in the experimental group penetrated into the cytoplasm through the cell membrane and deposited granularly around the mitochondria or selectively entered the mitochondria; lanthanum particles were deposited in the capillary bile duct lumen and the tight junctions and the hepatocyte interstices, And liver cell gap also see the abnormal alkaline phosphatase reactants. Ultrastructure shows mitochondria swelling, membrane destruction and endoplasmic reticulum dilatation. The results show that the target unit of shock wave is membrane structure and mitochondria. The early damage effect is the increase of permeability of the membranous membrane structure and the loss of completeness, leading to the change of subcellular structure and biochemical abnormalities.