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目的：对自制抗人雌激素受体Ｄ段合成肽（ｈＥＲＤ）的单克隆抗体（ＭｃＡｂ）进行Ｗｅｓｔｅｒｎ免疫印迹特异性分析，显示自制单抗与野生型ＥＲ有特异性反应。方法：对５１例乳腺癌组织石蜡切片或冰冻切片进行免疫组化（ＩＨＣ）检测，并与葡聚糖包裹活性炭吸附法（ＤＣＣ）进行比较，符合率９２％（４７／５１）。间接荧光免疫反应检测自制抗ＥＲ单抗对ＭＣＦ－７培养细胞的荧光标记为阳性，以已知ＥＲ检测值的三个不同石蜡组织为抗原，鉴定自制单抗可否用于临床流式细胞术（ＦＣＭ）检测。结果：其ＥＲ的阳性标记率及荧光道数均与已知ＩＨＣ检测结果相符。结论：提示自制单抗也可用于流式免疫学方法检测ＥＲ。各组实验均与进口ｈＥＲＡ／ＢＭｃＡｂ进行比较，结果相符。
OBJECTIVE: Western blot analysis of monoclonal antibodies (McAbs) against homemade human anti-human estrogen receptor D segment synthetic peptides (hERD) shows that the monoclonal antibodies produced by the monoclonal antibodies specifically react with wild-type ER. Methods: Immunohistochemistry (IHC) was performed on paraffin sections or frozen sections of 51 cases of breast cancer and compared with dextran coated activated carbon (DCC), the coincidence rate was 92% (47/51). Indirect fluorescence immunoassay detection of self-made anti-ER monoclonal antibody on MCF-7 cultured cells were positive for fluorescence labeling, known ER detection value of the three different paraffin tissue antigen, whether homemade monoclonal antibody for clinical flow cytometry ( FCM) test. Results: The positive rate of ER and the number of fluorescent channels were consistent with the known IHC results. Conclusion: It is suggested that homemade monoclonal antibody can also be used to detect ER by flow cytometry. Each group of experiments were compared with imported hERA / BMcAb, the results are consistent.