目的采用低剂量多柔比星诱导,建立耐多柔比星的S180的荷瘤小鼠模型。方法建立腹水型S180荷瘤小鼠模型。对荷瘤小鼠反复低剂量应用多柔比星,逐步诱导产生耐多柔比星的S180荷瘤小鼠模型。并通过多柔比星对S180杀伤率、流式细胞术动态检测MDR表达情况等评价模型建立情况。结果实验组小鼠自用药后1周MDR表达逐渐增高,自第5周后显著性增加(P<0.05),第7周的P-gp表达率是第1周的近10倍。第5周时实验组MDR阳性表达小鼠占66.7%,第7周占77.8%。多柔比星对S180杀伤率逐月降低。经传代后腹水型小鼠S180细胞的表达稳定。结论本方法简单方便,可用于在体肿瘤多药耐药研究。 Objective To establish a tumor-bearing mouse model of S180 with resistance to doxorubicin induced by low dose of doxorubicine. Methods Ascites S180 tumor-bearing mice model was established. Repeated low dose of doxorubicin in tumor-bearing mice, and gradually induced S180 tumor-bearing mouse model of resistance to doxorubicin. And through doxorubicin S180 killing rate, flow cytometry dynamic detection of MDR expression evaluation model to establish the situation. Results The expression of MDR in the experimental group increased gradually 1 week after the drug administration, significantly increased from the 5th week (P <0.05), and the expression rate of P-gp in the 7th week was nearly 10 times of the 1st week. At the fifth week, the MDR positive mice in the experimental group accounted for 66.7%, and the seventh week accounted for 77.8%. Doxorubicin S180 killing rate decreased month by month. After passage, the expression of S180 cells in ascites mouse was stable. Conclusion This method is simple and convenient and can be used in multidrug resistance studies in vivo.