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蛋白质和粗脂肪含量是评价稻米营养品质的重要指标,控制水稻(Oryza sativa L.)糙米蛋白质及脂肪含量的基因位点是数量性状,检测水稻糙米蛋白质及脂肪含量的数量性状位点(QTL)对于水稻品质遗传育种具有重要的意义。通过明恢63和优质泰国香米KDML105两个籼稻品种为亲本杂交的重组自交系(Recombinant inbred line,RIL)群体构建了包含113个简单重复序列标记(SSR)的遗传连锁图谱,对2009、2010年群体的蛋白质和粗脂肪含量进行了QTL定位。2009年检测到5个QTLs,其中蛋白质含量检测到2个QTLs,单个QTL贡献率分为别为5.44%和5.52%;粗脂肪含量检测到3个QTLs,单个QTL贡献率为5.42%～7.30%。2010年蛋白质含量检测到3个QTLs,单个QTL可解释表型变异为6.24%～20.75%;未检测到粗脂肪含量QTL。此外,还检测到14对粗脂肪含量和8对蛋白质含量的上位性QTLs。
The content of protein and crude fat is an important index to evaluate the nutritional quality of rice. The genetic loci controlling the protein and fat content of brown rice are quantitative traits, the quantitative trait locus (QTL) for detecting brown rice protein and fat content, It is of great significance for genetic breeding of rice quality. A genetic linkage map containing 113 simple repeat markers (SSRs) was constructed by using a recombinant inbred line (RIL) population derived from two indica rice varieties Minghui63 and high quality Thai KDML105. The genetic linkage maps of 2009, 2010 QTL mapping of protein and crude fat content in the population. Five QTLs were detected in 2009, of which two QTLs were detected in protein content, and the contribution rate of single QTL was 5.44% and 5.52% respectively; three QTLs were detected in crude fat content, and the contribution rate of single QTL was 5.42% -7.30% . Three QTLs were detected for protein content in 2010, and a single QTL could explain the phenotypic variation from 6.24% to 20.75%. No QTL for crude fat content was detected. In addition, 14 epistatic QTLs were detected for crude fat content and 8 pairs of protein content.