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目的:探讨白细胞介素(IL)－1β参与缺氧缺血性脑损伤(HIBD)的发病机制.方法:通过结扎左颈总动脉制备左脑HIBD的新生大鼠模型.用反转录聚合酶链反应(RT－PCR)技术检测新生大鼠HIBD后不同时间海马、皮质IL－1βMRNA的表达,经凝胶成像及分析系统扫描RT－PCR扩增产物,以内参照半定量分析IL－1βMRNA的动态变化.系列稀释的总RNA经RT－PCR扩增后行SOUTHERN杂交鉴定产物特异性.结果:RT－PCR技术敏感特异,HIBD后1HIL－1βMRNA开始增高,其高峰表达时间为HIBD后3H,并很快消失,高峰表达时损伤侧IL－1βMR－NA水平为健侧的4.92倍,P＜0.01,海马表达略高于皮质,P＞0.05.结论:IL－1β在发育期HIBD发病过程中可能起重要作用.“,”Objective: This paper was to study the role of interleukin 1(IL 1) β in the pathogenesis of hypoxia ischemia brain damage (HIBD). Methods: A model of HIBD in neonate rats was established. We monitored the IL 1 β mRNA in the lesioned ischemic cortex and hippocampus by reverse transcription polymerase chain reaction (RT PCR) at different phases after HIBD. Semiquintitive analysis of RT PCR products was performed by White UV Transilluminator. Southern blot hybridization was used to identify the specifici...