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1982年春和1983年春对斯帕坦(Spartan)苹果树进行了PP333(Paclobutrazol,一种赤霉素生物合成抑制剂)处理。在1983年,PP333处理者,其延长梢和短果枝的生长受到抑制,叶片数量及叶片面积均减少,果实体积和重量稍变小,新梢叶片和短果枝叶片的叶绿素含量未受影响,采收时果实的硬度和可溶性固形物未发生变化。在四次(盛花后78、112、134和163天)取样测定中,PP333处理者,其叶片的山梨糖醇含量显著增高,果糖、葡萄糖和蔗糖的含量几乎没有变化,淀粉的含量增高,果肉的碳水化合物含量无变化。在1984年春的各次取样测定中,虽然处理者的芽和枝条中的碳水化合物含量不同,但在芽的发育期间,各类碳水化合物的代谢作用似乎未受影响。处理对短果枝叶片的矿质元素含量也无影响。在盛花后112和134天的取样测定中,处理者的新梢叶片的钙含量显著增加。PP333处理对果肉和果皮中的矿质含量没有影响。
The spring of 1982 and the spring of 1983 were treated with paclobutrazol (a gibberellin biosynthesis inhibitor) against the Spartan apple tree. In 1983, the growth of shoots and short shoots of PP333 was inhibited, the number of leaves and the area of leaves were reduced, the volume and weight of fruit slightly decreased, the chlorophyll content of shoots and short branches was not affected, Fruit firmness and soluble solids did not change at harvest. In the four sampling tests (78, 112, 134 and 163 days after flowering), the content of sorbitol in leaves of PP333 treated plants was significantly increased, the content of fructose, glucose and sucrose hardly changed, the content of starch increased, No change in carbohydrate content. In the various sampling assays of the spring of 1984, the carbohydrate metabolism of various carbohydrates did not appear to be affected during bud development, although the carbohydrate content of the shoots and shoots of the processors differed. The treatment also had no effect on the mineral element content of short fruit branches. In the sampling assays at 112 and 134 days after flowering, the calcium content of the shooter shoot tip increased significantly. PP333 treatment had no effect on mineral content in pulp and peel.