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目的探讨丹参酮IIA磺酸钠注射液对肢体缺血再灌注损伤的保护机制,并观察丹参酮IIA的保护效应。方法健康成年SD大鼠60只,随机分为模型组,肢体缺血再灌注组,丹参酮IIA干预组,每组各20只。通过不同的分组实验,测定血清乳酸脱氧酶(LDH)、肌酸肌酶(CK)、肌红蛋白(Mb)的含量;以及后腿骨骼肌组织中丙二醛(MDA)、黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)的含量。结果再灌注后丹参酮IIA干预组LDH明显低于肢体缺血再灌注组,差异有统计学意义(P均<0.05)。骨骼肌组织SOD丹参酮IIA干预组较模型组及肢体缺血再灌注组均升高(P均<0.05),XOD、MDA则较模型组、肢体缺血再灌注组明显减少(P<0.05)。结论丹参酮IIA对肢体缺血再灌注损伤有明显治疗作用,其作用与清除氧自由肌抗氧化有关。
Objective To investigate the protective mechanism of sodium tanshinone IIA sulfonate injection on limb ischemia-reperfusion injury and to observe the protective effect of tanshinone IIA. Methods Sixty adult SD rats were randomly divided into model group, limb ischemia-reperfusion group and tanshinone IIA intervention group, with 20 rats in each group. Serum levels of lactate dehydrogenase (LDH), creatine kinase (CK) and myoglobin (Mb) were determined by different grouping experiments. The content of malondialdehyde (MDA), xanthine oxidase XOD), superoxide dismutase (SOD) content. Results The LDH of tanshinone IIA intervention group after reperfusion was significantly lower than that of limb ischemia reperfusion group (all P <0.05). Skeletal muscle SOD tanshinone IIA intervention group than the model group and limb ischemia reperfusion group were increased (P <0.05), XOD, MDA than the model group, limb ischemia reperfusion group was significantly reduced (P <0.05). Conclusion Tanshinone IIA has a significant therapeutic effect on limb ischemia-reperfusion injury, and its role is related to the elimination of oxygen free radicals muscle antioxidant.