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以木纤维为基质，与甲基丙烯酸环氧丙酯共聚接枝合成了复合膜介质，用复合膜介质制备了ｐｒｏｔｅｉｎＡ亲合膜色谱柱，考察了ｐｒｏｔｅｉｎＡ亲合膜色谱柱液相流动特性和吸附性能。实验证明：流速与亲合膜色谱柱柱压呈线性关系，当流速为３ｍＬ／ｍｉｎ时，柱压为１６０ｋＰａ。免疫球蛋白（ＩｇＧ）浓度和上样速度是影响ｐｒｏｔｅｉｎＡ键合容量的重要因素，对其进行了优化研究。用动态吸附法确定了对人ＩｇＧ动态最大吸附能力可达２１．７ｍｇ／ｇ（干介质）。通过对人血浆中ＩｇＧ的分离纯化考察了ｐｒｏｔｅｉｎＡ亲合膜色谱柱的分离能力，用毛细管电泳对纯化人ＩｇＧ的纯度进行了分析，证明一步纯化ＩｇＧ纯度可达９８％以上。亲合膜色谱柱分离速度快，操作简单，吸附容量满足要求，有望在免疫球蛋白的大规模分离纯化中得到应用。
The composite membrane was synthesized by copolymerization of wood fiber and glycidyl methacrylate. The proteinA affinity membrane column was prepared by using composite membrane medium. The flow characteristics and adsorption of proteinA affinity membrane column were investigated. performance. The experimental results show that the flow rate is linear with the column pressure of the affinity membrane. When the flow rate is 3 mL / min, the column pressure is 160 kPa. Immunoglobulin (IgG) concentration and loading rate are important factors that affect the protein A binding capacity, and have been optimized. Dynamic adsorption method to determine the maximum dynamic adsorption of human IgG up to 21.7mg / g (dry medium). The purity of purified human IgG was analyzed by capillary electrophoresis. The purification purity of proteinA affinity membrane column was 98%. Affinity membrane column separation speed, simple operation, adsorption capacity to meet the requirements, is expected to immunoglobulin large-scale separation and purification applications.