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目的探讨青霉素全身给药建立的大鼠癫痫模型在癫痫发作不同时间不同脑区的T2弛豫时间的改变。材料与方法成年健康雄性SD大鼠10只,腹腔注射青霉素300万U/kg体重×7天,分别于青霉素注射前、注射结束当日及注射后7天行MR成像,并对顶叶运动皮层、颞叶皮层、梨状皮层、齿状回、海马及侧脑室区域进行T2弛豫时间测量。结果青霉素全身给药建立的癫痫模型大鼠的行为表现以强直和阵挛为主。青霉素注射结束当日及注射后7天部分模型大鼠侧脑室区域在T2WI上出现异常信号;T2弛豫时间测量结果显示,青霉素注射结束当日及注射后7天双侧海马CA1区和侧脑室区域的T2弛豫时间没有明显差异,但相对于注射前有明显的差异(P<0.05)。T2WI检测侧脑室区域信号增强,组织学检测证实为侧脑室扩大。结论癫痫模型中脑局部区域出现T2弛豫时间明显变化,这些区域可能是导致癫痫发作或与癫痫发作有关的重要神经生物区域。
Objective To investigate the changes of T2 relaxation time in different brain regions of rats with epileptic seizures induced by penicillin. MATERIALS AND METHODS Ten adult male Sprague Dawley rats were injected intraperitoneally with penicillin 3 million U / kg body weight × 7 days. MR imaging was performed before penicillin injection, on the injection end and 7 days after injection, respectively. Tissue of temporal cortex, piriform cortex, dentate gyrus, hippocampus and lateral ventricle were measured for T2 relaxation time. Results The performance of epilepsy model rats established by systemic administration of penicillin was dominated by tonic and clonus. On the day of penicillin injection and 7 days after injection, abnormal signals were found on T2WI in part of model rats. T2 relaxation time measurement showed that on the day of penicillin injection and on the 7th day after injection, bilateral hippocampal CA1 area and lateral ventricle area There was no significant difference in T2 relaxation time, but significant difference compared with before injection (P <0.05). T2WI detection of lateral ventricle signal enhancement, histological examination confirmed lateral enlargement. Conclusions There is a significant change of T2 relaxation time in the middle cerebral region in the epilepsy model. These regions may be important neurobiological regions leading to seizures or seizures.