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本文作者运用不同的方法—筛选cDNA文库,克隆出表达于活化的t淋巴细胞的白细胞介素-13(IL-13),并将IL-13基因定位于人类第5号染色体5q23-31。 首先作者通过分化筛选的方法克隆出编码一种新的细胞因子的cDNA,此蛋白在1993年Keystore细胞因子专题讨论会上被命名为白细胞介素13(IL13),IL-13 cDNA是一个具有2个蛋氨酸起始的同源顺序CCA/GCCAUGG相连。推测出的IL-13多肽的氨基末端与其他分泌蛋白相似,亦呈疏水性,而
The authors used a different approach - screening cDNA libraries, cloning interleukin-13 (IL-13) expressed on activated t-lymphocytes and locating the IL-13 gene on human chromosome 5 5q23-31. First, the authors cloned a cDNA encoding a novel cytokine by the method of differentiation and selection. This protein was named interleukin-13 (IL13) at the Keystore cytokine symposium in 1993. IL-13 cDNA is a cDNA with 2 A homologous sequence of methionine initiation CCA / GCCAUGG. The putative amino-terminus of the IL-13 polypeptide resembles other secreted proteins and is also hydrophobic