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白细胞移动特别是趋化运动在机体的免疫功能中超重要作用,因而对白细胞移动进行研究具有重要意义。检测白细胞移动的方法有体内法和体外法两大类。迄今为止,体外法中应用得最广泛的仍是六十年代初期Boyden所创始的微孔滤膜法和七十年代中期Nelson等所建立的琼脂糖下玻片法。琼脂糖下玻片法在国内已有人采用,而微孔滤膜法迄今尚未见国内文献记载。本文报道用微孔滤膜法检测26名正常成年人外周血中性粒细胞移动的结果。在中性粒细胞三种类型的移动中,我们只检测了随机移动(random migration)和趋化运动(Chemotaxis),而对化学促动性(Chemokinesis)未进行研究。
Leukocyte movement, especially chemotaxis, plays an important role in the immune function of the body, and therefore it is of great significance to study leukocyte movement. Leukocyte detection methods are in vivo and in vitro methods of two categories. To date, the most widely used in vitro methods are the microporous membrane method pioneered by Boyden in the early 1960s and the agarose slide method established by Nelson in the mid-1970s. The agarose slide method has been used in China, and the microporous membrane method has not yet been found in the domestic literature. This article reports the results of the detection of neutrophil migration in peripheral blood of 26 normal adults using a microporous membrane method. Of the three types of neutrophil movement, we only examined random migration and chemotaxis, while we did not study for Chemokinesis.