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目的研究RANTES基因启动子区-403G/A、-28C/G和内含子区Inl.1T/C的多态性和结核病易感性之间的关系。方法采用病例对照研究方法,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对78例结核病患者(病例组)和97例健康对照者(对照组)的RANTES-403G/A、-28C/G和Inl.1T/C多态性进行基因分型,分析这三个位点多态性与结核病发生率的关系。结果 2组RANTES-403G/A位点GG、GA和AA基因型的分布频率比较差异无统计学意义(x2=0.41,P>0.05),G、A等位基因分布频率差异无统计学意义(x2=0.30,P>0.05)。2组RANTES-28C/G位点CC、CG和GG基因型的分布频率比较差异无统计学意义(x2=0.15,P>0.05),C、G等位基因分布频率差异无统计学意义(x2=0.02,P>0.05)。2组RANTES Inl.1T/C位点TT、TC和CC基因型的分布频率比较差异无统计学意义(x2=0.53,P>0.05),T、C等位基因分布频率差异无统计学意义(x2=0.19,P>0.05)。结论 RANTES基因启动子区的-403G/A、-28C/G和内含子区的Inl.1T/C位点的单核苷酸多态性(SNP)与肺结核感染的易感性不相关。
Objective To investigate the relationship between the polymorphism of Inl.1T / C at -403G / A, -28C / G and the intron of RANTES gene and the susceptibility to tuberculosis. Methods A case-control study was conducted in 78 TB patients (case group) and 97 healthy controls (control group) using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for RANTES-403G / A , -28C / G and Inl.1T / C polymorphisms were genotyped and analyzed the relationship between the three polymorphisms and the incidence of tuberculosis. Results There was no significant difference in the distribution frequencies of GG, GA and AA genotypes between RANTES-403G / A loci in the two groups (x2 = 0.41, P> 0.05). There was no significant difference in the distribution frequencies of G and A alleles x2 = 0.30, P> 0.05). There was no significant difference in genotype frequencies of CC, CG and GG between the two groups (χ2 = 0.15, P> 0.05), but there was no significant difference in genotype frequencies of C and G = 0.02, P> 0.05). There was no significant difference in the distribution frequencies of TT, TC and CC genotypes between RANTES Inl.1T / C sites (χ2 = 0.53, P> 0.05), but there was no significant difference in the distribution frequencies of T and C alleles x2 = 0.19, P> 0.05). Conclusion The single nucleotide polymorphisms (SNP) at -403G / A, -28C / G and IN1.1T / C loci in the RANTES gene promoter region are not correlated with the susceptibility to tuberculosis infection.