论文部分内容阅读
该工作以富含大量胸腺嘧啶(Thymine,T)核酸单链为识别分子,SYBR Green I(SG)为荧光基团,建立了一种简单、灵敏的荧光增强法检测Hg2+。由于T-Hg2+-T键的形成,富T单链自我折叠或者两两配对形成双链DNA结构,当溶液中的SG嵌入DNA双链中时,SG荧光强度显著增强。实验结果表明,SG荧光强度随着Hg2+浓度的增加而增加。在最优实验条件下,SG的荧光强度与Hg2+的浓度在4.000×10-7~2.000×10-6mol/L范围内呈线性关系,检出限为3.900×10-8 mol/L。该方法在含5.0%湘江水实际样品中获得的回收率为98.72%~104.5%,因此该传感器可用于实际湘江水样品中Hg2+的测量。
In this work, a simple and sensitive fluorescence enhancement method was developed for the detection of Hg2 + with SYBR Green I (SG) as a recognition molecule, which contains a large number of single-stranded nucleic acid thymine (T) as a recognition molecule. Due to the formation of the T-Hg2 + -T bond, the T-rich single-stranded self-folding or pairwise pairing forms a double-stranded DNA structure. When the SG in solution is embedded in the double-stranded DNA, the SG fluorescence intensity is significantly enhanced. The experimental results show that SG fluorescence intensity increases with the increase of Hg2 + concentration. Under the optimal experimental conditions, the fluorescence intensity of SG was linear with the concentration of Hg2 + in the range of 4.000 × 10-7 ~ 2.000 × 10-6mol / L with the detection limit of 3.900 × 10-8 mol / L. The recovery rate of this method in 98% of Xiangjiang River water samples was 98.72% ~ 104.5%, so the sensor could be used to measure Hg2 + in the actual Xiangjiang water samples.