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硝基左旋精氨酸甲基酯(N—nitro—L—arginine methyl ester,L—NAME)是一种一氧化氮合成酶的特异性抑制剂。本实验选用BALB/c小鼠,~(60)Coγ-射线全身一次性照射12Gy后,再给予L—NAME灌胃,于照后72h处死。应用ND组化、H—E染色及Feulgen反应肠腺计数方法,对小肠壁的NOS阳性结构、小肠的组织结构及肠腺存活率等进行观察。结果:与单纯照射组相比,接受L—NAME灌胃的小鼠,其小肠扩张、充血程度明显降低,肠腺存活率明显提高(P<0.01),肠粘膜组织结构明显改善,肌间神经丛内NOS阳性细胞密度及染色深度明显降低,肠腺腔及肠绒毛表面的NOS阳性产物亦减少。本文结果提示:肠壁NOS活性增强加重了小肠组织的放射损伤,应用L-NAME对受照小鼠小肠具有一定程度的保护作用。
Nitro-L-arginine methyl ester (N-nitro-L-arginine methyl ester, L-NAME) is a specific inhibitor of nitric oxide synthase. In this experiment, BALB / c mice were selected and irradiated with ~ (60) Coγ-ray once a single dose of 12Gy, followed by intragastric administration of L-NAME and sacrificed at 72h after irradiation. Application of ND staining, H-E staining and Feulgen reaction intestinal gland counting method, the NOS positive structure of small intestine, intestinal tissue structure and intestinal gland survival rate were observed. Results: Compared with the irradiation group, the mice fed with L-NAME had a significant reduction in the small intestine expansion and hyperemia, significantly increased the survival of the gut (P <0.01), and significantly improved the structure of the intestinal mucosa The density and depth of NOS-positive cells in the plexus were significantly decreased, and the positive products of NOS in the intestinal gland lumen and intestinal villi also decreased. Our results suggest that increased intestinal NOS activity aggravates the radiation damage in the small intestine, and the L-NAME protects the irradiated small intestine to a certain extent.