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以高灌蓝莓品种‘都克’试管苗移栽到田间6个月的植株为试材,研究了高温胁迫条件下,外源一氧化氮(NO)对高灌蓝莓幼苗生长、PSⅡ光化学活性和抗氧化系统的影响.结果表明:0.2、0.5和1.0 mmol.L-1外源NO供体硝普钠(SNP)处理显著减缓了高温胁迫对高灌蓝莓光合系统Ⅱ(PSⅡ)的抑制,高灌蓝莓PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)和非光化学猝灭系数(NPQ)下降缓慢,使光合机构免受高温胁迫的伤害;叶片质膜透性和丙二醛含量降低,而超氧化物歧化酶和过氧化氢酶活性升高,从而促进了脯氨酸的积累.适当浓度的SNP能显著缓解高温胁迫对高灌蓝莓植株的伤害,其中0.5 mmol.L-1 SNP的缓解效果最好.
The experiment was conducted to study the effects of exogenous nitric oxide (NO) on the growth, PSⅡ photochemical activity and the activity of PSⅡ in high-intensity shrubs under high temperature stress The results showed that 0.2, 0.5 and 1.0 mmol.L-1 exogenous nitric oxide donor nitroprusside (SNP) significantly slowed down the inhibition of high temperature stress on photosystem Ⅱ (PSⅡ) Fv / Fm, actual photochemical efficiency (ΦPSⅡ), photochemical quenching coefficient (qP) and non-photochemical quenching index (NPQ) of blueberry decreased slowly and the photosynthetic system was protected from high temperature stress. Membrane permeability and malondialdehyde content decreased, while superoxide dismutase and catalase activity increased, which promoted the accumulation of proline.The appropriate concentration of SNP can significantly alleviate high temperature stress on high-irrigation blueberry plant damage , Of which 0.5 mmol.L-1 SNP had the best alleviation effect.