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小麦农家品种ZB90是一个广谱抗白粉病材料。苗期和成株期鉴定表明,其白粉病抗性由显性单基因控制。从位于不同染色体上的53对微卫星引物中筛选出3对引物(Wms382、Wms311和Wms312),对144株F2分离群体进行抗病连锁性分析。另外,与Pm4a共分离的STS标记STS-470也被用于基因定位。这4个标记和抗病基因在染色体上的顺序为:基因位点-Xgwm382-Xgwm311-STS-470-Xgwm312,它们之间的遗传距离分别为2.9、4.5、23.1和49.6 cM,与小麦染色体2AL上已构建的微卫星图谱顺序一致。根据材料来源和抗病基因的染色体定位结果,确定该基因为一个新的小麦抗白粉病基因,并命名为PmZB90。文中还讨论了PmZB90和其他位于染色体2AL上的基因之间的关系。
ZB90, a wheat farm cultivar, is a broad spectrum powdery mildew resistant material. Seedling and adult stage identification showed that the powdery mildew resistance was controlled by dominant single gene. Three pairs of primers (Wms382, Wms311 and Wms312) were screened from 53 pairs of microsatellite primers located on different chromosomes. Resistance analysis of 144 F2 isolates was carried out. In addition, STS marker STS-470 co-segregated with Pm4a was also used for gene mapping. The sequence of the four markers and the resistance genes on the chromosomes was as follows: the locus -Xgwm382-Xgwm311-STS-470-Xgwm312, with the genetic distances of 2.9, 4.5, 23.1 and 49.6 cM, respectively, Constructed microsatellite maps in the same order. According to the chromosomal location of the material source and disease resistance gene, the gene was identified as a new wheat powdery mildew resistance gene and named PmZB90. The paper also discusses the relationship between PmZB90 and other genes located on chromosome 2AL.