以新疆主栽陆地棉品种为实验材料,通过花粉管通道法导入盐穗木耐盐相关基因HcNHX1和HcVP1。经过连续两代筛选繁育,结果显示:外源基因已经整合进棉花受体材料基因组中,并且在RNA水平有表达;外源基因对T1代棉花转基因植株生长影响很大,表现为生长势弱,植株矮小,株铃少,但T2代的棉花转基因植株在株高、果枝数和株铃等方面均有不同程度的恢复。 Xinjiang Upland cotton cultivars were used as experimental materials to introduce salt-tolerance genes HcNHX1 and HcVP1 through pollen tube pathway. After two generations of selection and breeding, the results showed that the exogenous gene has been integrated into the genome of cotton receptor material and expressed at the level of RNA. The exogenous gene has a great influence on the growth of T1 transgenic cotton plants, showing weak growth potential, The plants were short and the number of bolls was small. However, the T2 generation of cotton transgenic plants recovered to varying degrees in plant height, fruit branch number and bell.