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目的定量测定血小板反应素-4(TSP-4)基因在胃癌及癌旁组织中的表达,探讨其与胃癌临床病理学特征、微血管密度(MVD)和基质金属蛋白酶-9(MMP-9)表达水平的关系,评价其在胃癌发生和发展中的意义。方法采用RT-PCR技术检测82例配对的胃癌组织及癌旁组织TSP-4mRNA的表达,并分析其与临床病理学特征的关系;通过免疫组织化学检测肿瘤组织中CD34和MMP-9的表达,分析TSP-4mRNA的表达与肿瘤微血管密度、MMP-9表达的相关性。结果TSP-4mRNA在胃癌中的表达高于癌旁组织(P=0.03);胃癌组织中TSP-4mRNA的表达水平与肿瘤大小、组织分型、淋巴结转移、肿瘤微血管密度及MMP-9表达密切相关(P=0.002,P=0.031,P=0.014,r=0.67P<0.01,P=0.008),但与性别、年龄及侵袭程度无关(P=0.53,P=0.57,P=0.15)。结论TSP-4可能通过调节胃癌新血管生成和MMP-9的表达,促进肿瘤生长与转移。
Objective To quantitatively determine the expression of thrombospondin-4 (TSP-4) gene in gastric cancer and paracancerous tissues and to investigate the relationship between the expression of TSP-4 gene and the clinicopathological features, microvessel density (MVD) and the expression of matrix metalloproteinase-9 Level relationship, evaluate its significance in the occurrence and development of gastric cancer. Methods RT-PCR was used to detect the expression of TSP-4 mRNA in 82 paired gastric cancer tissues and paracancerous tissues. The relationship between TSP-4 mRNA and clinical pathological features was analyzed. The expressions of CD34 and MMP-9 in tumor tissue were detected by immunohistochemistry, TSP-4 mRNA expression and tumor microvessel density, MMP-9 expression was analyzed. Results The expression of TSP-4 mRNA in gastric cancer tissues was significantly higher than that in adjacent tissues (P = 0.03). The expression of TSP-4 mRNA in gastric cancer tissues was closely related to the tumor size, histological type, lymph node metastasis, tumor microvessel density and MMP- (P = 0.002, P = 0.031, P = 0.014, r = 0.67P <0.01, P = 0.008), but not with gender, age and invasion (P = 0.53, P = 0.57, P = 0.15). Conclusion TSP-4 may promote tumor growth and metastasis by regulating the expression of MMP-9 and neovascularization in gastric cancer.