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目的：为了探讨快速准确地进行肾移植供受体的ＨＬＡ分型。方法：采用单克隆抗体免疫磁珠法分离Ｔ、Ｂ淋巴细胞；以软加法技术加样后，将反应板置于 ３７℃孵箱中孵育 ５０ｍｉｎ。结果：免疫磁珠分离出的 Ｔ、Ｂ细胞纯度均为１００％，细胞活性 ９６％以上；软加法可避免相邻孔间反应的相互污染，分型结果易于指定；提高反应温度、缩短反应时间，获得以原方法一致的分型结果。结论：采用免疫磁珠分离Ｔ、Ｂ淋巴细胞、以软加法技术加样、提高反应温度、缩短反应时间，有助于准确快速地进行ＨＬＡ分型。
OBJECTIVE: To investigate the rapid and accurate HLA typing of renal recipients for recipients. Methods: T and B lymphocytes were isolated by monoclonal antibody immunomagnetic beads method. After adding samples by soft addition technique, the plate was incubated in a 37 ℃ incubator for 50 min. Results: The purity of T and B cells isolated by immunomagnetic beads were 100% and their cell viability was above 96%. The soft addition could avoid the mutual contamination of adjacent wells and the typing results could be easily specified. The reaction temperature and the reaction time were shortened , Obtained by the original method of consistent typing results. CONCLUSION: T and B lymphocytes are separated by immunomagnetic beads and loaded with soft addition technique to increase the reaction temperature and shorten the reaction time, which is helpful for the accurate and rapid HLA typing.