AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver.METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine,theplasmid could be expressed in eukaryotic cellswhen injected into Wistar rats by intravenousroute.At different time intervals after the injection,the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry.RESULTS The expression of the plasmid bindingto liposomes or G-PLL could be markedly observed24 h later,and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively,but the effect of thelatter was better than the former concerning thedistribution and expression of the plasmid targeteduptake in the liver.CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver. AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat liver. METHODS After binding with lipofectamine orgalactose-terminal glyco-poly-L-lysine, theplasmid could be expressed in eukaryotic cells injected into Wistar rats by intravenous route. At different time intervals after the injection, the distribution and expression of the plasmid inliver of rats were observed and compared using insitu hybridization and immunohistochemistry. RESULTS The expression of the plasmid binding to liposomes or G-PLL could be observed observed 24 h later, and began to decrease one week later, but it it could could be observed up to three weeks.Both liposomes and G-PLL could deliver theplasmid to the liver effectively, but the effect of thelatter was better than the former concerning the distribution and expression of the plasmid targeted uptake in the liver. CONCLUSION G-PLL is better than liposome asthe targeted carrier for delivering exogenous genesto the liver