研究了氧氟沙星与四氰基乙烯 (TCNE)的荷移反应 ,确立了形成电荷转移 (CT)络合物的最佳条件。在丙酮介质中 ,于 50℃水浴中恒温 30 min即可形成 1∶ 1络合物 ,其最大吸收波长为 40 9nm。表观摩尔吸光系数 ε为 2 .8× 1 0 4 L· mol- 1· cm- 1。在 0～ 1 2 μg· m L- 1范围内符合比耳定律。方法的相对标准偏差小于 1 %。对形成 CT络合物的机理进行了探讨。用拟定的方法对样品氧氟沙星药片进行测定 ,结果与紫外分光光度法一致 ,回收率符合要求 The charge-transfer reaction between ofloxacin and tetracyanoethylene (TCNE) was studied and the optimal conditions for the formation of charge-transfer (CT) complexes were established. In acetone medium, a 1: 1 complex was formed by isothermal incubation at 50 ° C for 30 min in a water bath with a maximum absorption wavelength of 40 9 nm. The apparent molar absorptivity ε is 2.8 × 10 4 L · mol -1 · cm -1. Beer’s law is in the range of 0 ~ 1 2 μg · m L -1. The relative standard deviation of the method is less than 1%. The formation of CT complex mechanism was discussed. The proposed method for the determination of ofloxacin tablets, the results and UV spectrophotometry consistent, the recovery rate to meet the requirements