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目的研究人外周血淋巴细胞(PBMCs)体外诱导前后的蛋白质组表达变化,进一步探讨其抗肿瘤机制。方法收集培养前后的细胞提取总蛋白,定量、双向电泳和银染后,利用ImageMasterTM软件对表达异同的蛋白质点进行分析及质谱鉴定。结果培养前后细胞相似的蛋白主要与基因转录因子和细胞骨架相关,差异蛋白主要与细胞凋亡和代谢相关。结论 CIK细胞的扩增及表型变化与细胞内蛋白表达改变相关。
Objective To study the changes of proteome expression of human peripheral blood lymphocytes (PBMCs) before and after induction in vitro, and to further explore its anti-tumor mechanism. Methods The total protein was extracted from the cells before and after culture. The protein spots were analyzed by ImageMasterTM software and identified by mass spectrometry after two-dimensional electrophoresis and silver staining. Results The cell-like proteins before and after culture were mainly related to gene transcription factors and cytoskeleton. The differential proteins were mainly associated with apoptosis and metabolism. Conclusion CIK cell proliferation and phenotypic changes correlate with changes in intracellular protein expression.