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苹果果胶多糖经脱酯化得到果胶酸,以果胶酸为底物通过酶解得到聚合度为2～8的果胶寡糖混合物,并运用阴离子交换柱层析对果胶寡糖混合物进行了分离制备.结果表明,以果胶酸为底物在40℃酶解反应1h为最佳酶解条件得到果胶寡糖混合物,经分离制备得到聚合度为2～5的寡聚半乳糖醛酸单体,总得率为37%,TLC和ESI-MS检测其纯度为92%以上.运用ESI-MS分析寡聚半乳糖醛酸时发现除单体的分子离子峰外,还存在二聚体的分子离子峰,并对二聚体的存在进行了确认。
The pectic acid was obtained by de-esterification of apple pectin polysaccharides, pectic acid oligosaccharides with degree of polymerization of 2-8 were obtained by enzymatic hydrolysis using pectic acid as substrate, and the pectic oligosaccharide mixture was separated by anion exchange chromatography Were isolated and prepared.The results showed that pectic acid oligosaccharides mixture was obtained by enzymatic hydrolysis at 40 ℃ for 1h, and the oligosaccharides with degree of polymerization of 2 ~ 5 The total yield of aldehyde monomer was 37%, and the purity of the product was over 92% by TLC and ESI-MS.Using ESI-MS analysis of oligomeric galacturonic acid, it was found that in addition to the monomer molecular ion peak, The molecular ion peak of the body was confirmed by the presence of the dimer.