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目的 了解国内献血人群中一种新的经血传播病毒 (SENV )感染的流行状况。 方法 以 SENV ORF1区核苷酸序列设计引物建立套式聚合酶链反应 (n PCR)方法。采用多重 PCR法对 5 96份来自 3个不同地区无偿和 /或有偿献血者标本进行 SENV DNA(D和 H亚型 )检测 ,并对 PCR阳性产物进行克隆后测序分析。 结果 在体检合格的献血者中 ,SENV DNA检出率为 13 .5 %～ 2 1.0 % ;在抗 -HCV、HBs Ag、抗 -HIV、梅毒抗体阳性和 ALT异常升高的献血者中 ,SENV DNA检出率分别为 3 5 .0 %、14 .0 %、60 .0 %、2 8.6%和 3 1.3 % ;献血者中 SENV-D亚型感染率高于 SENV-H亚型 ;不同地域献血者 SENV DNA检出率的差异无显著性 (P>0 .0 5 ) ,体检不合格献血者 (抗 -HIV或抗 -HCV阳性者的 SENV-D感染率显著高于正常献血人群 (P<0 .0 5 ) ;6份来自不同个体和不同地域之间的 SENV分离株部分基因组核苷酸的变异最高达 11.9% ,与标准株 (AX0 2 5 83 8)相比变异高达 13 .2 %。 结论 在国内献血人群中存在 SENV感染
Objective To understand the prevalence of a new SENV infection among blood donors in China. Methods Nested PCR was designed based on the nucleotide sequence of ORF1 in SENV. Multiplex PCR was used to detect SENV DNA (D and H subtypes) in 5 96 gratuitous and / or paid blood donors from 3 different regions, and the PCR positive products were cloned and sequenced. Results The positive rate of SENV DNA was 13.5% ~ 2 1.0% among the blood donors who passed the examination. Among the blood donors who were positive for anti-HCV, HBsAg, anti-HIV, syphilis antibodies and abnormally elevated ALT, SENV The detection rate of DNA was 35.0%, 14.0%, 60.0%, 22.6% and 31.3% respectively. The infection rate of SENV-D subtype in blood donors was higher than that of SENV-H subtype. There was no significant difference in the detection rate of SENV DNA between donors and donors (P> 0.05). The prevalence of SENV-D infection was significantly higher in patients with unqualified blood donors than in normal blood donors <0. 05). The variation of some genomic nucleotides in 6 SENV isolates from different individuals and different regions was up to 11.9%, which was as high as 13.2 as compared with the standard strain (AX0 2 5 83 8) %. Conclusion SENV infection exists in blood donors in China