7α,15α-二羟基雄烯醇酮1是合成口服避孕药Yasmin活性成分屈螺酮的重要中间体,应用亚麻刺盘孢(Colletotrichum lini AS 3.4486)的羟化酶系统对去氢表雄酮底物进行生物转化,合成了7α,15α-二羟基雄烯醇酮,转化率60～70％。转化机理研究发现,亚麻刺盘孢将底物的7位羟基化合成7α-羟基雄烯醇酮,再将其15位羟基化形成双羟基的产物。亚麻刺盘孢菌能够特异性、有序地完成对去氢表雄酮的两步羟化反应,与文献报道的结论一致,间接转化方法和转化机理属于首次报道。 7α, 15α-dihydroxyandrostenolone 1 is an important intermediate for the synthesis of drospirenone, the active ingredient of the oral contraceptive Yasmin. Hydroxygenase system of Colletotrichum lini AS 3.4486 was used to treat dehydroepiandrosterone end Biotransformation, synthesis of 7α, 15α-dihydroxy androstenolone, the conversion rate of 60 to 70%. Transformation mechanism study found that flax spore substrate 7 hydroxylation of 7α-hydroxy androstenolone, and then its hydroxylation of 15 to form the product of the dihydroxy. Flaxseed can specifically and orderly complete the two-step hydroxylation of dehydroepiandrosterone, which is consistent with the reported results in the literature. Indirect transformation and transformation mechanisms are the first reported reports.