论文部分内容阅读
目的了解影响儿童注意缺陷多动障碍(ADHD)发生的多巴胺羟化酶(DβH)基因特定位点多态性及相关环境影响因素间的关系,为综合干预ADHD的发生提供科学依据。方法选取包头市市区及郊区各1所小学,对全体在校生进行流行病学调查,筛查并诊断出ADHD儿童104名,选取同校其他同性别年龄相差<2岁的相互间无血缘关系的健康儿童104名为对照,分析环境影响因素、DβH基因特定位点多态性及二者之间交互作用与ADHD的关系。结果 Logistic回归分析显示,年级(OR=0.622)和家庭知识性(OR=0.747)是ADHD发生的保护因素,而父亲惩罚、严厉(OR=1.138)和父亲偏爱被试(OR=1.140)是危险因素,DβH5’侧翼区-1021C/T-CC基因型是ADHD发生的危险因素(OR=2.040)(P值均<0.05)。基因多态性检测显示,ADHD组与对照组DβH5’侧翼区-1021C/T基因型分布比较差异无统计学意义(χ~2=2.297,P=0.130),两组等位基因频率比较差异无统计学意义(χ~2=0.019,P=0.780)。基因-环境交互作用结果显示,父亲惩罚、严厉与DβH5’侧翼区-1021C/T-CC基因型发生存在负向交互作用(r=0.357,OReg=1.047,P=0.027),父亲偏爱被试与DβH5’侧翼区-1021C/T-CC基因型发生存在负向交互作用(r=0.557,OReg=1.076,P=0.035)。结论特定位点基因型与特定环境因素间的交互作用可能影响ADHD的发生。
Objective To investigate the relationship between polymorphism of dopamine hydroxylase (DβH) gene and related environmental factors in children with ADHD and to provide a scientific basis for the comprehensive intervention of ADHD. Methods One primary school in Baotou urban and suburban areas was selected. Epidemiological survey was conducted among all the students in the study, and 104 children with ADHD were screened and diagnosed. Other unrelated children Healthy children 104 as control, analysis of environmental factors, DβH gene-specific site polymorphisms and the interaction between the two and ADHD. Results Logistic regression analysis showed that grade (OR = 0.622) and family knowledge (OR = 0.747) were the protective factors of ADHD. However, father punishment, severe (OR = 1.138) and father preference (OR = 1.140) Factor, DβH5 ’flanking region -1021C / T-CC genotype is a risk factor for ADHD (OR = 2.040) (P all <0.05). The results of gene polymorphism showed that the distribution of -1021C / T genotypes in DβH5 ’flanking region was not significantly different between ADHD group and control group (χ ~ 2 = 2.297, P = 0.130). There was no significant difference in allele frequencies between the two groups Statistical significance (χ ~ 2 = 0.019, P = 0.780). The results of gene-environment interaction showed that there was a negative interaction between father’s punishment and severity and -1021C / T-CC genotype of DβH5 ’(r = 0.357, OReg = 1.047, P = 0.027) There was a negative interaction between -1021C / T-CC genotype of DβH5 ’flanking region (r = 0.557, OReg = 1.076, P = 0.035). Conclusion The interaction between specific locus genotypes and specific environmental factors may affect the occurrence of ADHD.