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在特定培养条件下对６２个陆地棉（ＧｏｓｙｐｉｕｍｈｉｒｓｕｔｕｍＬ．）品种进行了离体培养研究。结果表明，基因型对愈伤组织的诱导率没有明显影响，但对体细胞胚胎发生能力影响很大。仅２７．４％的品种有体细胞胚胎发生能力，其中胚胎发生能力较强的品种占１１．３％，多数品种（７２．６％）在本试验中未观察到胚状体形成。在含有ＩＡＡ及ＫＴ的培养条件下，基因型对愈伤组织的生长速度有明显影响。双列杂交结果表明，基因型间愈伤组织生长速度和体细胞胚胎发生能力存在显著差异。愈伤组织生长速度的一般配合力、特殊配合力和反交效应都达到极显著水平。无胚胎发生能力的品种与有胚胎发生能力的品种杂交所得２０个组合都不能形成胚状体，且Ｆ２代呈３∶１的分离比例，表明体细胞胚胎发生受一对隐性主效基因控制。在有胚胎发生能力的基因型中，胚胎发生能力还存在量的差异，这种差异可能受一些修饰基因的控制。
62 cultivars of Gossypium hirsutum L. were cultured in vitro under specific culture conditions. The results showed that the genotype had no significant effect on the callus induction rate, but had a great effect on the somatic embryogenesis ability. Only 27.4% of the cultivars had somatic embryogenesis, 11.3% of them were embryo-producing, and most of the cultivars (72.6%) did not observe embryogenesis in this experiment. Genotypes had a significant effect on the callus growth rate under IAA and KT culture conditions. The results of the two-line hybridization showed that the callus growth rate and somatic embryogenesis ability of genotypes were significantly different. The general combining ability, special combining ability and anti-cross effect of callus growth rate reached extremely significant level. None of the 20 combinations that hybridized with an embryo-producing variety and an embryogenic-producing variety did not form an embryoid body and the F2 generation showed a 3: 1 segregation ratio indicating that somatic embryogenesis was controlled by a pair of recessive major genes . In embryo-producing genotypes, there are also differences in embryogenesis capacity, which may be controlled by some modified genes.