论文部分内容阅读
以外源性腺苷前质硫酸腺嘌呤大鼠心脏预处理和经典缺血预处理建立模型，监测心脏内源性保护物质的变化。方法：采用２５０～３００ｇＳＤ大鼠３２只分成４组，即假手术组（ＳＯ组）、缺血再灌组（Ｉ／Ｒ组）、经典缺血预处理组（ＩＰＣ组）及硫酸腺嘌呤预处理组（ＡＳＰＣ组）。比较各组心肌内源性保护物质的变化。结果：ＩＰＣ组、ＡＳＰＣ组均显示缩小心梗面积、改善心功能的ＩＰＣ效应，同时，肌酸激酶（ＣＫ）、过氧化氢酶（ＣＡＴ）、５”核苷酸酶（５’ＮＴ）、超氧化物歧化酶（ＳＯＤ）活性增强以及一氧化氮（ＮＯ）含量增高，热休克蛋白７０（ＨＳＰ７０）ｍＲＮＡ表达增强。结论：ＣＫ、ＣＡＴ、５’ＮＴ、ＳＯＤ、ＮＯ及ＨＳＰ７０是大鼠腺苷性和缺血性心脏预处理后产生的重要的内源性保护物质。
The model of exogenous adenosine preconditioning-adenosine sulphate-induced rat cardiac preconditioning and classical ischemic preconditioning was established to monitor the changes of cardiac endogenous protective substances. Methods: Thirty-two SD rats (250-300 g SD) were divided into four groups: sham operation group (SO group), ischemia reperfusion group (I / R group), classical ischemic preconditioning group (IPC group) and adenine sulphate Treatment group (ASPC group). The changes of endogenous protective substances in each group were compared. Results: IPC group and ASPC group showed IPC effect of reducing myocardial infarction area and improving cardiac function. At the same time, the activity of CK, CAT, 5 "nucleotidase ), Superoxide dismutase (SOD) activity and nitric oxide (NO) content increased, HSP70 mRNA expression increased.Conclusion: CK, CAT, 5’NT, SOD, NO and HSP70 Important endogenous protective substances produced after rat adenosine and ischemic preconditioning.