水蛭是传统中药,它的主要药效功能之一是具有抗凝血酶活性组分。而在中药材及其制剂中能否检测出具有抗凝血酶活性,除与药材制取方法和制剂制备的工艺有关外,还可能与所含有的伪水蛭素含量有关。本文报道应用凝血酶偶联带有氨基末端的磁性微粒,从两种水蛭干品浸出液中分离纯化水蛭素,通过对抗凝血酶活性的检测,以求取对药材质量的评价,本法工艺简单、快速,亦可用于批量生产的初步纯化,为进一步精制提供基础。 Leech is a traditional Chinese medicine, and one of its main medicinal functions is to have an antithrombin activity component. The ability to detect the presence of antithrombin activity in Chinese herbal medicines and their preparations may be related to the content of pseudo-hirudin contained in addition to the preparation methods of the medicinal materials and the preparation of the preparations. In this paper, the use of thrombin coupled with amino-terminated magnetic particles to separate and purify hirudin from the leech extracts of two leech extracts was used to evaluate the antithrombin activity to evaluate the quality of the medicinal materials. This method is simple, Fast, can also be used for preliminary purification of batch production, providing the basis for further refining.