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随着免疫组织化学方法的发展,认识到分布在中耳粘膜和咽鼓管的植物神经除肾上腺素能神经、胆硷能神经外,还有神经递质即各种神经肽也是一种具有植物神经样作用的物质。著者们用萤光抗体法(FITC法)及可溶性酶——抗酶组化法(PAP法)观察了豚鼠中耳粘膜内P物质(SP)、血管运动性肠多肽(VIP)、鸟胰多肽(APP)、促胃液素释放肽(GRP)等四种神经肽的分布情况,以及咽鼓管内SP的分布其结果是:在中耳粘膜SP、VIP、APP神经纤维较多而GRP神经纤维较少。神经肽纤维多沿血管走行,但也可见独立走行的纤维。神经肽纤维在咽鼓管开口部多,离开口部越远越少这与山下
With the development of immunohistochemical methods, recognizing the distribution in the middle ear mucosa and eustachian tube nerve in addition to adrenergic nerve, cholinergic nerve, but also neurotransmitter that is a variety of neuropeptides is also a plant Nerve-like substance. The authors observed the changes of substance P (SP), vasoactive intestinal polypeptide (VIP), and bird pancreatic polypeptide in the middle ear mucosa of guinea pigs by using the fluorescent antibody method (FITC method) and the enzyme- (APP), gastrin-releasing peptide (GRP) and other neuropeptides, and the distribution of SP in the eustachian tube. The results showed that there were more SP, VIP and APP nerve fibers in the middle ear mucosa and more GRP nerve fibers less. Neuropeptide fibers travel along the blood vessels, but can also see the independent walking fibers. Neuropeptide fibers in the eustachian tube opening more, leaving the mouth farther and farther this mountain