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水稻细菌性条斑病(Xanthomonas campe-stris PY.oryzicola)是国内病害检疫对象。近年在国内危害严重,专家普遍认为此病通过带菌稻种的调运造成蔓延。甘薯瘟病(Pseudomonassolanacearum)在我国南方主要薯区危害严重,薯苗是传病的主要途径。以上两种病害目前均无特异性强、灵敏度高的检测方法,尤其水稻细菌性条斑病,种子带菌无法检测,研究应用单克隆抗体检测技术测定带菌将解决生产上一大难题。1987年以来,分别从病叶上分离、纯化水稻细菌性条斑病菌和甘薯瘟病菌作免疫原,以我们研究改进的方法对杂交瘤细胞进行筛选,获得具有极强特异性的
Rice bacterial leaf streak (Xanthomonas campe-stris PY.oryzicola) is the object of domestic disease quarantine. In recent years, serious harm in the country, experts generally believe that the disease caused by the transfer of carriers of rice caused by spread. Pseudomonas solanacearum is very harmful to the main potato areas in southern China. Potato seedlings are the main pathways of disease transmission. At present, there is no specific and sensitive detection method for the above two diseases, especially the bacterial leaf streak of rice and the seed carrying bacteria can not be detected. The study of detection of carrier bacteria by monoclonal antibody testing technology will solve a production difficult problem. Since 1987, isolated from the diseased leaves, respectively, Purification of rice bacterial leaf stool fungus and Magnaporthe grisea as immunogen, with our improved method of screening hybridoma cells to obtain highly specific