论文部分内容阅读
Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro.A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed.Contents of transforming growth factor (TGF)-β1,interleukin(IL)-6 and marx metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA).In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages.In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P＜0.05).Levels of TGF-β 1,IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated,compared with control group (P＜0.05).In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-1 0 secretion at different incubation time,but it did not affect collagen secretion of fibroblasts.Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages,and increased its tumor necrosis factor (TNF)-α secretion.In conclusion,carboxymethyl-chitosan promoted wound healing by activating macrophages,accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines.