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对来自欧洲和美国的 18个刺槐种源子代进行了等位酶分析。可进行遗传分析的 7个酶系统 (Amy,Fe,L ap,Idh,Mdh,6 Pgd,Skd)中有 14个基因位点 ,其中 12个位点具有多态性。每个多态位点平均等位基因数 (A/L )变化在 1.5 6～ 3.6 7之间 ,平均基因型数 (G/L)变化在 1.6 1～ 7.11之间 ,平均等位基因有效数目 (Ae)变化在 1.0 2～ 2 .5 0之间 ,预期杂合度 (H e)变化在0 .0 2～ 0 .5 6之间。不同种源群体之间也存在较大的遗传差异 ,在 8个德国种源中 ,各群体的 A、Ae、和 H e等相对较小 ,但不同群体间差异较大。各位点等位基因频率在不同种源群体间变化也较大 ,表明德国各种源群体内遗传变异相对较小 ,但群体间差异较大。来自匈牙利和斯洛伐克的 8个种源群则相反 ,各群体的 A、Ae、和 H e等相对较大 ,而不同种源群体间差异则较小 ,各位点等位基因频率在种源群体间变化相对一致 ,表明这两个国家的种源群体内变异较大 ,但不同种源群体间差异较小。欧洲的刺槐种源并未形成明显的地理变异模式 ,而且欧洲的种源和来自原产地的美国种源相比 ,没有发现明显的差异。经过 Hardy-Weinberg平衡检测证明 ,88.4 1%位点符合 H- W遗传平衡 ,表明各群体基因频率和基因型频率保持较高的稳定性 ,且种源内的变异大于种源间变异 ,94 .
Allozyme analyzes of 18 locust sources from Europe and the United States were performed. Seven of the seven enzyme systems (Amy, Fe, L ap, Idh, Mdh, 6 Pgd, Skd) that could be genetically analyzed had 14 loci, of which 12 were polymorphic. The average number of alleles per polymorphic locus (A / L) varied from 1.56 to 3.67 and the average genotype (G / L) varied from 1.6 to 7.11. The average number of alleles per locus (Ae) varied from 1.02 to 2.5. The expected heterozygosity (He) varied from 0.2 to 0.56. There are also large genetic differences among different provenance populations. Among the eight German provenances, A, Ae, and H e of each population are relatively small, but there are significant differences among different groups. The frequency of alleles at different loci varied greatly among different provenance populations, indicating that the genetic variations among various source populations in Germany were relatively small, but the differences among populations differed greatly. The eight provenances from Hungary and Slovakia are opposite. The A, Ae, and H e of each population are relatively large, while the differences among different provenances are small. The allele frequency of each locus The changes are relatively consistent, indicating that the two countries have more variation within the provenance population, but the differences between the different provenance populations are smaller. Acacia species in Europe did not form a clear pattern of geographical variation, and the European provenance and provenance from the United States compared to the provenance did not find significant differences. After Hardy-Weinberg equilibrium test, 88.4% of the loci were in accordance with the H-W genetic balance, indicating that the genetic frequency and genotype frequency of each population maintained high stability, and the variation within the provenance was greater than that between the provenances.