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以1,2-苯并-3,4二氢咔唑-9-乙基氯甲酸酯(BCEOC)作为柱前衍生试剂,采用非水毛细管电泳对衍生小肽进行分离。在甲醇乙腈混合液为溶剂,乙酸-乙酸铵为缓冲体系,20℃,28kV,235nm二极管阵列检测(DAD)条件下,采用非水毛细管电泳模式,考察并优化小肽衍生物的分离条件,实现四种小肽衍生物的基线分离。
The small peptides were separated by non-aqueous capillary electrophoresis with 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as a pre-column derivatization reagent. Under the conditions of 20 ℃, 28kV and 235nm diode array detection (DAD), the separation conditions of the small peptide derivatives were investigated and optimized by using the non-aqueous capillary electrophoresis mode in a mixture of methanol and acetonitrile as solvent and acetic acid-ammonium acetate as buffer system. Baseline Separation of Four Small Peptide Derivatives.