论文部分内容阅读
目的研究火箭液体推进剂偏二甲基肼(UDMH)和四氧化二氮(N2O4)吸入性急性肺损伤(ALI)中肺泡表面活性物质(PS)的变化及其与ALI的关系。方法84只大鼠分为对照组和5个实验组。实验组在静式染毒柜内分别吸入UDMH(0·98g/m3)和N2O4(0·19g/m3)各10min,间隔10min。实验组在吸入后2、6、12、24、48h分别处死。测定各组大鼠常压下支气管肺泡灌洗液(BALF)的表面张力(γ)、加压下最低表面张力(γmin)、磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)。同时测定肺组织湿/干重比(W/D)、BALF中总蛋白和乳酸脱氢酶(LDH)等肺损伤指标,评价病理学改变。结果UDMH-N2O4吸入性ALI表现为肺W/D比值增加、BALF中总蛋白和LDH升高,以及肺水肿、肺间隔增厚等病理变化。PS的异常表现为:①在UDMH-N2O4吸入后2hBALF中γ和γmin即明显升高,至24~48h尚未恢复到对照组水平;②吸入后24hBALF中PC明显低于对照组,PE含量明显高于对照组。以BALF中总蛋白作为肺损伤指标与PS异常的指标γ和γmin分别做直线相关分析,相关系数分别为0·435(P<0·01)和0·419(P<0·01)。结论UDMH-N2O4吸入性ALI中存在PS异常,PS异常可能参与了UDMH-N2O4吸入性ALI的发生发展过程。
Objective To investigate the changes of alveolar surfactant (PS) and its relationship with ALI in rocket liquid propellant UDMH and nitrous oxide (N2O4) -induced acute lung injury (ALI). Methods 84 rats were divided into control group and 5 experimental groups. The experimental group inhaled UDMH (0 · 98g / m3) and N2O4 (0 · 19g / m3) for 10min in 10min interval in 10min interval. The experimental group were sacrificed at 2,6,12,24,48h after inhalation. The surface tension (γ), the lowest surface tension (γmin), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in bronchoalveolar lavage fluid (BALF) under normal pressure were measured. The wet / dry weight ratio (W / D) of lung tissue, total protein in lung tissue and LDH were measured to evaluate the pathological changes. Results Inhaled ALI in UDMH-N2O4 showed an increase in W / D ratio of the lung, increased total protein and LDH in the BALF, and pathological changes such as pulmonary edema and thickening of the lung space. The abnormalities of PS were as follows: (1) The γ and γmin of BALF in 2h after inhalation of UDMH-N2O4 were significantly increased, but the level of control group did not recover to the level of 24-48h after inhalation of UDMH-N2O4; the PC of 24hBALF after inhalation were significantly lower than that of control group In the control group. Linear correlation analysis was made between total protein in BALF and γand γmin, which were indicators of lung injury and PS abnormality respectively. The correlation coefficients were 0.43 (P <0.01) and 0.41 (P <0.01) respectively. Conclusions There is abnormal PS in inhaled ALI in UDMH-N2O4. PS abnormality may be involved in the development and progression of inhaled ALI in UDMH-N2O4.