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目的 分析SARS IgG、SARS IgM、核壳蛋白 IgG抗体和核壳蛋白抗原的变化特点并探讨其意义。方法 采用酶联免疫方法对 2 0 0 3年末至 2 0 0 4年初新发 4例SARS病人的系列血清进行上述 4项的滴度检测。结果 在病人较早期的血清中可以检测到核壳蛋白抗原 ,随着抗体水平的升高 ,抗原含量迅速下降。抗体上升下降快 ,在最高水平维持时间短 ,并且除第 1例外 ,其余 3例的抗体滴度均小于 1∶1 0 0 ,核壳蛋白抗体变化规律与总的IgG抗体一致 ,但滴度更低。结论 抗体变化规律与前次流行不同 ,抗体水平变化快 ,应注意及时采集标本。抗原检测可作为一种实验室诊断依据。核壳蛋白抗体可考虑用于早期诊断。
Objective To analyze the characteristics of SARS IgG, SARS IgM, nucleocapsid protein IgG antibody and nucleocapsid protein antigen and to explore its significance. Methods Serum samples of 4 SARS patients newly diagnosed from the end of 2003 to the beginning of 2004 were tested by enzyme-linked immunosorbent assay. As a result, nucleocapsid antigen was detected in the early serum of patients, and antigen levels decreased rapidly with the increase of antibody level. Antibody up and down faster, at the highest level to maintain a short time, and in addition to the first exception, the remaining three cases of antibody titers were less than 1: 1 0 0, nucleocapsid protein antibody changes consistent with the total IgG antibodies, but the titers more low. Conclusions Antibody variation is different from previous epidemic. Antibody level changes rapidly. Antigen detection can be used as a laboratory diagnosis. Nucleocapsid protein antibodies are considered for early diagnosis.