Employing the orthogonal design,the optimal extraction conditions for flavonoid in leaves of black currant were determined by colorimetric estimation,which were extraction agent of 75% EtOH－H2O,the material ratio (ratio of weight of fresh leaves to volume of the solvent)of 1:16, the temperature of 35 ℃, the time of 1 h and times of three. A total extraction rate of over 97% and the fiavonoid contents of 179. 33 mg/100 g were obtained under the optimal extraction conditions.