BALB/c小鼠骨髓瘤P_3×63Ag8细胞与同系小鼠经Vero细胞传代的麻疹病毒LEC株免疫的脾细胞在PEG作用下杂交。杂交细胞分种微量培养盘的孔内,进行孵育,用HAT培养液筛选单克隆细胞,用RIA检测,单克隆细胞孔中60％麻疹抗体阳性。 28个杂交瘤细胞系接种BALB/c小鼠腹腔内,经3～5周,从每只小鼠腹腔抽取2～10ml腹水,测定其麻疹抗体活性,其抗体滴度较原培养液高20～200倍。28个杂交瘤的 BALB / c mouse myeloma P_3 × 63Ag8 cells and splenocytes immunized with syngeneic mice measles virus LEC strain passaged by Vero cells were hybridized under the action of PEG. The hybrid cells were subcultured in the wells of a microplate and incubated. Monoclonal cells were screened in HAT medium and detected by RIA. 60% of the measles antibodies in the wells of monoclonal cells were positive. 28 hybridoma cell lines were inoculated BALB / c mice peritoneal cavity, after 3 to 5 weeks, per mouse peritoneal cavity aspiration 2 ~ 10ml, measles antibody activity was measured, the antibody titers higher than the original culture medium 20 ~ 200 times. 28 hybridomas