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目的:研究高脂饮食对大鼠血管内皮细胞衰老的影响。方法:大鼠高脂饲料喂养12周后测定血脂的变化,HE染色分析胸主动脉和肾动脉血管结构的改变;胶原酶灌注方法分离胸主动脉和肾动脉血管内皮细胞,应用免疫细胞化学的方法鉴定内皮细胞;应用细胞化学染色的方法检测衰老相关的半乳糖甘酶(senescence-associated β-galactosidase,SA-β-gal)活性。结果:SD雄性大鼠高脂饲料喂养12周后,大鼠血浆三酰甘油、总胆固醇、高密度脂蛋白胆固醇水平明显升高,胸主动脉和肾动脉出现明显的粥样硬化改变;胸主动脉和肾动脉血管内皮细胞SA-β-gal活性明显增加,表现为较强的β-gal染色。结论:高脂饮食引起大鼠胸主动脉和肾动脉粥样硬化改变,动脉血管内皮细胞衰老增加。
Objective: To study the effect of high-fat diet on the senescence of rat vascular endothelial cells. Methods: The changes of blood lipid in rats fed with high-fat diet for 12 weeks were measured. The structure of thoracic aorta and renal artery was analyzed by HE staining. The thoracic aorta and renal artery endothelial cells were separated by collagenase perfusion method. Immunocytochemistry Methods The endothelial cells were identified. The senescence-associated β-galactosidase (SA-β-gal) activity was detected by cytochemical staining. Results: After fed with high-fat diet for 12 weeks, the levels of triglyceride, total cholesterol and high-density lipoprotein cholesterol in SD rats increased significantly, and the atherosclerosis in the thoracic aorta and renal arteries changed significantly. Arterial and renal artery endothelial cells SA-β-gal activity was significantly increased, showed strong β-gal staining. Conclusion: The high-fat diet induces the change of atherosclerosis in thoracic aorta and renal artery, and the aging of arterial endothelial cells.