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目的:探讨生长抑素受体(SSTR)1～3亚型蛋白在大肠癌组织中表达及其与临床病理学的关系。方法:收集41例大肠癌患者手术切除肿瘤组织标本及23例远癌组织(指距肿瘤活检切除边缘3～6 cm的组织)标本,采用免疫组化PV-9000二步法检测其SSTR1～3亚型蛋白的表达;采用Fisher确切概率法进行统计学分析,并分析与临床相关因素的关系。结果:41例大肠癌组织中SSTR1～3亚型蛋白的阳性表达率分别为65.8%(27/41)、61.0%(25/41)和56.1%(23/41),均较远癌组织表达率低(P<0.05)。SSTR1～3亚型蛋白在大肠癌组织中的表达与患者的性别、有无淋巴结转移和临床分期无关,SSTR2和SSTR3亚型蛋白与肿瘤的分化程度及肿瘤的部位无关;而SSTR1亚型蛋白在高/高中分化的大肠癌组织中的表达高于低中分化和低分化大肠癌组织中的表达,且直肠癌中的表达较结肠癌低(P<0.05)。结论:SSTR1亚型蛋白是大肠癌组织中的主要表达形式,可能与大肠癌的发生发展有关。
Objective: To investigate the expression of somatostatin receptor (SSTR) subtype 1 ~ 3 in colorectal carcinoma and its relationship with clinicopathology. METHODS: Totally 41 specimens of colorectal cancer were collected for surgical resection of tumor tissues and 23 specimens of distant tissues (3 ~ 6 cm away from tumor biopsy margin). Immunohistochemistry PV-9000 two-step method was used to detect the expression of SSTR1-3 Subtypes of protein expression; using Fisher’s exact test for statistical analysis and analysis of the relationship with clinical factors. Results: The positive expression rates of SSTR1 ~ 3 subtypes in 41 cases of colorectal cancer were 65.8% (27/41), 61.0% (25/41) and 56.1% (23/41), respectively, The rate was low (P <0.05). The expression of SSTR1 ~ 3 subtype in colorectal carcinoma was not related to the patient’s gender, lymph node metastasis and clinical stage. The SSTR2 and SSTR3 subtypes were not related to tumor differentiation and tumor location. However, The expression of high / high differentiated colorectal cancer tissues was higher than that in low and medium differentiated and poorly differentiated colorectal cancer tissues, and the expression in colorectal cancer was lower than that in colorectal cancer (P <0.05). Conclusion: SSTR1 isoform is the main expression in colorectal cancer tissues, which may be related to the occurrence and development of colorectal cancer.